Functional assay for GABA receptor subtypes of a cockroach giant interneuron
Identifieur interne : 001419 ( Main/Exploration ); précédent : 001418; suivant : 001420Functional assay for GABA receptor subtypes of a cockroach giant interneuron
Auteurs : G. Hue [France]Source :
- Archives of Insect Biochemistry and Physiology [ 0739-4462 ] ; 1991.
English descriptors
- KwdEn :
Abstract
γ ‐ Aminobutyric acid (GABA) receptors were examined in the cockroach central nervous system (CNS) using the single fiber‐oil gap method applied to an identified giant interneuron. Short‐lasting pressure application of 10 mM GABA developed a multiphasic response composed of a fast hyperpolarization followed by a transient depolarizing component and a stable hyperpolarization. This triphasic characteristic shape of the response was modified according to the dose of GABA injected or bath‐applied and to the precise localization of the injection within the dendritic area. The transient depolarizing phase showed a negative reversal potential of −70 mV. Both hyperpolarizing phases reversed at a more negative level ranging to −80 mV. A positive shift of these values was caused by a decrease in external chloride concentration. Bath‐application of 0.1 mM picrotoxin (Ptx) decreased the depolarizing phase which was progressively replaced by a stable hyperpolarization. The transient depolarizing component desensitized quickly and was the most sensitive phase to Ptx action. The Ptx‐resistant response reversed at a mean value of −100 mV close to the equilibrium potential for potassium ions (EK+), suggesting that it was generated by a K+‐channel coupled receptor. Although baclofen was unable to mimic the Ptx‐resistant GABA response, the compound CGA 147823, known to bind with a high specificity to vertebrate GABAB receptors, has been successfully used to reproduce the Ptx‐resistant GABA response. It is suggested that, in addition to GABA receptors linked to chloride channels, the insect CNS possesses GABA receptors sharing ionic characteristics of GABAB receptors especially those located in the vertebrate CNS, although they are insensitive to baclofen.
Url:
DOI: 10.1002/arch.940180303
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000A95
- to stream Istex, to step Curation: 000690
- to stream Istex, to step Checkpoint: 000C22
- to stream Main, to step Merge: 001472
- to stream Main, to step Curation: 001419
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Functional assay for GABA receptor subtypes of a cockroach giant interneuron</title><author><name sortKey="Hue, G" sort="Hue, G" uniqKey="Hue G" first="G." last="Hue">G. Hue</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:57604C487AC18DE25623BC3A34215BA477C602AB</idno><date when="1991" year="1991">1991</date><idno type="doi">10.1002/arch.940180303</idno><idno type="url">https://api.istex.fr/document/57604C487AC18DE25623BC3A34215BA477C602AB/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000A95</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000A95</idno><idno type="wicri:Area/Istex/Curation">000690</idno><idno type="wicri:Area/Istex/Checkpoint">000C22</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000C22</idno><idno type="wicri:doubleKey">0739-4462:1991:Hue G:functional:assay:for</idno><idno type="wicri:Area/Main/Merge">001472</idno><idno type="wicri:Area/Main/Curation">001419</idno><idno type="wicri:Area/Main/Exploration">001419</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Functional assay for GABA receptor subtypes of a cockroach giant interneuron</title><author><name sortKey="Hue, G" sort="Hue, G" uniqKey="Hue G" first="G." last="Hue">G. Hue</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Neurophysiologie, URA CNRS 611 Université d'Angers, 49045 Angers Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Pays de la Loire</region><settlement type="city">Angers</settlement></placeName><orgName type="university">Université d'Angers</orgName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Neurophysiologie, URA CNRS 611, Faculté de Médecine, rue haute de reculée, 49045 Angers Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Pays de la Loire</region><settlement type="city">Angers</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Insect Biochemistry and Physiology</title><title level="j" type="abbrev">Arch. Insect Biochem. Physiol.</title><idno type="ISSN">0739-4462</idno><idno type="eISSN">1520-6327</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1991">1991</date><biblScope unit="volume">18</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="147">147</biblScope><biblScope unit="page" to="157">157</biblScope></imprint><idno type="ISSN">0739-4462</idno></series><idno type="istex">57604C487AC18DE25623BC3A34215BA477C602AB</idno><idno type="DOI">10.1002/arch.940180303</idno><idno type="ArticleID">ARCH940180303</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0739-4462</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>fiber‐oil gap method</term><term>insect CNS</term><term>synaptic receptor</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">γ ‐ Aminobutyric acid (GABA) receptors were examined in the cockroach central nervous system (CNS) using the single fiber‐oil gap method applied to an identified giant interneuron. Short‐lasting pressure application of 10 mM GABA developed a multiphasic response composed of a fast hyperpolarization followed by a transient depolarizing component and a stable hyperpolarization. This triphasic characteristic shape of the response was modified according to the dose of GABA injected or bath‐applied and to the precise localization of the injection within the dendritic area. The transient depolarizing phase showed a negative reversal potential of −70 mV. Both hyperpolarizing phases reversed at a more negative level ranging to −80 mV. A positive shift of these values was caused by a decrease in external chloride concentration. Bath‐application of 0.1 mM picrotoxin (Ptx) decreased the depolarizing phase which was progressively replaced by a stable hyperpolarization. The transient depolarizing component desensitized quickly and was the most sensitive phase to Ptx action. The Ptx‐resistant response reversed at a mean value of −100 mV close to the equilibrium potential for potassium ions (EK+), suggesting that it was generated by a K+‐channel coupled receptor. Although baclofen was unable to mimic the Ptx‐resistant GABA response, the compound CGA 147823, known to bind with a high specificity to vertebrate GABAB receptors, has been successfully used to reproduce the Ptx‐resistant GABA response. It is suggested that, in addition to GABA receptors linked to chloride channels, the insect CNS possesses GABA receptors sharing ionic characteristics of GABAB receptors especially those located in the vertebrate CNS, although they are insensitive to baclofen.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Pays de la Loire</li></region><settlement><li>Angers</li></settlement><orgName><li>Université d'Angers</li></orgName></list><tree><country name="France"><region name="Pays de la Loire"><name sortKey="Hue, G" sort="Hue, G" uniqKey="Hue G" first="G." last="Hue">G. Hue</name></region><name sortKey="Hue, G" sort="Hue, G" uniqKey="Hue G" first="G." last="Hue">G. Hue</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/CobaltMaghrebV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001419 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001419 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= CobaltMaghrebV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:57604C487AC18DE25623BC3A34215BA477C602AB
|texte= Functional assay for GABA receptor subtypes of a cockroach giant interneuron
}}
| This area was generated with Dilib version V0.6.32. |  |